PURIFICATION OF THE ANTERIOR HYPOPHYSEAL THYROTROPIC HORMONE
نویسندگان
چکیده
منابع مشابه
The Intracellular Localization of Pituitary Thyrotropic Hormone
The intracellular localization of a bovine anterior pituitary preparation of thyroid-stimulating hormone (TSH) was studied in guinea pigs and dogs. The preparation was administered intravascularly or applied directly to tissue sections. TSH was detected by an indirect technique utilizing bovine TSH antiserum and fluorescein-labeled anti-rabbit globulin; the presence of TSH in the tissue was ind...
متن کاملPreparation of pituitary thyrotropic hormone.
The existence of a thyroid-stimulating hormone in anterior pituitary gland tissue has been known for 30 years (1, 2). During this time, considerable work has been done on the physiological and the pharmacological effects of the thyrotropic hormone administ’ered in the form of pituitary extracts which had been subjected t,o a varying degree of manipulation. Relatively little progress on the puri...
متن کاملPreparation of Pituitary Thyrotropic Hormone*
The existence of a thyroid-stimulating hormone in anterior pituitary gland tissue has been known for 30 years (1, 2). During this time, considerable work has been done on the physiological and the pharmacological effects of the thyrotropic hormone administ’ered in the form of pituitary extracts which had been subjected t,o a varying degree of manipulation. Relatively little progress on the puri...
متن کاملStarch-gel electrophoresis and chromatography in the purification of beef thyrotropic hormone.
Progress in the purification of the thyroid-stimulating hormone of the anterior pituitary has been slow, when compared to that of corticotropin, growth hormone, and prolactin, which are now well characterized. Earlier workers concentrated thyrotropic activity, which has been attributed to a glycoprotein, by a variety of extraction and precipitation procedures, and preparations whose potencies c...
متن کاملUltracentrifugation of hypophyseal growth hormone.
Sedimentation velocity was measured in a Spinco ultracentrifuge’ operating at 165,000 X g. The hormone solutions (0.5 per cent) were prepared with borate buffer of pH 9.7 or saline at pH 9.1. Table I presents a typical run in a borate buffer; the sedimentation velocity constant was calculated by the procedure described by Svedberg and Pedersen (3). It may be noted that the individual values of ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1955
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71067-3